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目的探索烟气的损伤特征,并建立烟气生物效应的快速检测新模型。方法采用脱细胞-核DNA作为实验模型,用烟气染毒,实验分烟气直接染毒(按照染毒时间分为:60、30、15和0min 4个组)和烟气水过滤后染毒(水过滤烟气、水过滤空气、烟的水溶液、空气的水溶液和水对照5个组),用彗星试验检测DNA损伤情况。结果烟气直接染毒的DNA损伤顺序为:60min组>30min组>15min组>0min组,呈现时间-反应关系;处理后烟气对DNA的损伤顺序为:烟气水溶液组>水过滤烟气>水过滤空气=空气水溶液=水溶液;直接染毒60min对DNA的损伤大于水过滤后烟气。结论烟气可直接损伤脱细胞DNA,水过滤后烟气对DNA的损伤降低;脱细胞-核DNA对烟气的损伤比较敏感,可用于烟气生物效应的评价。
Objective To explore the damage characteristics of flue gas and to establish a new rapid detection model of flue gas biological effects. Methods The acellular-nuclear DNA was used as experimental model. Flue gas was used to inoculate the cells. The experiment was divided into four groups (60, 30, 15 and 0 min according to the exposure time) Toxicity (water filtration flue gas, water filtration air, aqueous smoke, aqueous air and water controls) was used to detect DNA damage using comet assay. Results The sequence of DNA damage induced by direct exposure to flue gas was as follows: 60min group> 30min group> 15min group> 0min group, showing a time-response relationship. The damage order of DNA to flue gas after treatment was: flue gas aqueous solution group> water filtration flue gas > Air filtration air = air = aqueous solution of air; direct exposure to DNA damage for 60min greater than the filtered water flue gas. Conclusion The flue gas can directly damage the acellular DNA. The flue gas on water can reduce the DNA damage. The acellular-nuclear DNA is sensitive to the flue gas damage and can be used to evaluate the biological effects of flue gas.