论文部分内容阅读
目的探讨丙泊酚对缺氧无糖(OGD)损伤脑片的保护作用。方法采用缺氧无糖的人工脑脊液灌流离体大鼠海马脑片模拟缺血损伤,根据给药方式不同分为:OGD前给药组和OGD给药组,均给予1、10和20μmol·L-1的丙泊酚和脂肪乳(溶剂对照)。记录海马脑片顺向群峰电位(OPS)和缺氧损伤电位(HIP)的变化。结果OGD给药组应用10μmol·L-1的丙泊酚,与应用脂肪乳相比OPS消失时间推迟(P<0.05),OPS恢复率升高(P<0.05),HIP出现时间推迟(P<0.01),HIP出现率降低(P<0.05)。结论在缺氧过程中应用10μmol·L-1的丙泊酚对缺氧无糖损伤海马脑片具有保护作用。
Objective To investigate the protective effect of propofol on brain slices damaged by anoxic and sugar-free (OGD). Methods The hippocampal slices isolated from rat hippocampus were perfused with hypoxic and sugar-free artificial cerebrospinal fluid to simulate ischemic injury. According to the mode of administration, they were divided into two groups: pre-OGD group and OGD-administered group, with 1, 10 and 20 μmol·L -1 propofol and fat emulsion (solvent control). The change of forward peak potential (OPS) and hypoxic injury potential (HIP) in hippocampal slices were recorded. Results OGD administration group (10μmol·L-1 propofol) delayed OPS disappearance time (P <0.05), OPS recovery rate (P <0.05) and delayed HIP time (P < 0.01), HIP incidence decreased (P <0.05). Conclusion The application of 10μmol·L-1 propofol has a protective effect on hypoxic-glucose-deficient hippocampal slices during hypoxia.