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研究了我国主要经济红藻之一条斑紫菜藻红蛋白各种粗提方法。采用“溶胀+组织捣碎”法破碎条斑紫菜叶状体细胞,依次研究活性炭、利凡诺等化学试剂和等电点、溶液浓度、pH、温度、差速离心等物理条件对条斑紫菜破碎液中藻红蛋白纯度和产率的影响。结果显示:小量活性碳与利凡诺的结合使用不能提高藻红蛋白纯度,单独使用活性碳,随用量提高,其纯度上升,在活性碳用量是紫菜用量的1/10时提纯效果最佳。藻红蛋白等电点沉淀后纯度反而下降,但pH调节与硫酸铵沉淀结合可显著提升纯度,且与原溶液浓度成正比,藻红蛋白纯度最高达到1.35。此外,盐析过程中差速离心还可继续提升纯度,达到1.42,而通过控制温度来变性蛋白的方法不能达到提纯的效果。实验为进一步改进优化条斑紫菜藻红蛋白粗提做了铺垫。
This paper studied various crude extraction methods of Phycoerythrin, a major economic red algae in China. Using “swelling + Tissue mashed” method to break the leaf cells of Porphyra yezoensis, the chemical conditions such as activated carbon, Rivanol and other physical conditions such as isoelectric point, solution concentration, pH, temperature, differential centrifugation, Effects of phycoerythrin on the purity and yield of phycoerythrin in the broken seaweed. The results showed that the combination of a small amount of activated carbon with ricinol could not increase the purity of phycoerythrin. When the amount of activated carbon was used alone, the purity of phycoerythrin increased. . However, the purity of phycoerythrin decreases after isoelectric precipitation, but the combination of pH adjustment and ammonium sulfate precipitation can significantly improve the purity, and is proportional to the concentration of the original solution, the highest phycoerythrin purity reached 1.35. In addition, the differential centrifugation during the salting process can continue to increase the purity to 1.42, and the method of denaturing the protein by controlling the temperature can not achieve the purification effect. The experiment paved the way for further improvement and optimization of the crude extract of Phytoplankton Phycoerythrin.