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目的观察姜黄素作用于皮肤鳞状细胞癌SCL-1细胞后E-cadherin和p14ARF基因甲基化状态及基因表达的改变情况,为姜黄素的临床应用提供理论依据。方法用不同浓度(10μmol/L、20μmol/L、40μmol/L)的姜黄素处理皮肤鳞癌细胞株SCL-1,MTT法检测细胞增殖情况,甲基化特异性PCR(MSP)法检测E-cadherin和p14ARF基因甲基化状态的改变,RT-PCR法检测E-cadherin和p14ARF基因的表达情况。结果姜黄素可抑制SCL-1细胞的增殖,且呈现时间-剂量依懒性;当姜黄素浓度增至40μmol/L时,SCL-1细胞的E-cadherin和p14ARF基因甲基化减弱、表达增强。结论姜黄素可明显抑制SCL-1细胞增殖,适当浓度的姜黄素对SCL-1细胞的E-cadherin和p14ARF基因有一定的去甲基化作用,并可以调控E-cadherin和p14ARF基因的表达。
Objective To observe the changes of methylation status and gene expression of E-cadherin and p14ARF after SCT-1 cells treated with curcumin, and provide a theoretical basis for the clinical application of curcumin. Methods SCL-1 cells were treated with curcumin at different concentration (10μmol / L, 20μmol / L, 40μmol / L), MTT assay was used to detect cell proliferation, methylation specific PCR (MSP) cadherin and p14ARF gene methylation status changes, RT-PCR method to detect E-cadherin and p14ARF gene expression. Results Curcumin inhibited the proliferation of SCL-1 cells in a dose-dependent and time-dependent manner. When the concentration of curcumin was increased to 40 μmol / L, the methylation of E-cadherin and p14ARF in SCL-1 cells decreased and the expression of . Conclusion Curcumin can significantly inhibit the proliferation of SCL-1 cells. Appropriate concentration of curcumin can demethylate E-cadherin and p14ARF genes in SCL-1 cells and regulate the expression of E-cadherin and p14ARF.