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为了探究模拟微重力对新生乳鼠海马脑区的影响,将新生1 d SD(Sprague Dawley)鼠的海马脑区分别做组织块培养和原代细胞培养,将培养物随机分为模拟微重力组1 d组和地面对照组。回转1 d后,用苏木精-伊红染色法(HE染色)、显微镜观察等方式检测组织块表面及内部形态变化,并用流式细胞术检测原代细胞的凋亡情况。通过明场和相差显微镜观察到与对照组相比,组织块经过1 d的模拟微重力刺激后,其厚度发生变化,组织块边缘变薄,透光度增加;HE染色结果显示,与对照组相比,模拟微重力组组织块内部细胞分布散乱。流式细胞仪检测结果显示,原代细胞在经过1 d的模拟微重力刺激后,其细胞凋亡率与对照组相比无显著差异。以上实验结果说明,经过1 d的模拟微重力后,海马组织块的表面和内部形态发生了较大变化,但凋亡率和地面对照组的相比无显著差异。
To investigate the effects of simulated microgravity on hippocampal brain regions in neonatal neonatal rats, cultured hippocampal brain regions of neonatal SD rats were randomly divided into simulated microgravity group 1 d group and ground control group. After 1 d of rotation, the surface and internal morphology of the tissue were examined by hematoxylin-eosin staining (HE staining) and microscopic observation. The apoptosis of primary cells was detected by flow cytometry. Compared with the control group, the thickness of the tissue block changed after simulated microgravity stimulation for 1 day, and the edge of the tissue block became thinner and the light transmittance increased. The results of HE staining showed that compared with the control group Compared with the simulated microgravity group, the distribution of internal cells was disorganized. Flow cytometry results showed that primary cells after 1 d of simulated microgravity stimulation, the apoptosis rate compared with the control group no significant difference. The above experimental results show that after 1 d of simulated microgravity, the surface and internal morphology of hippocampal tissue blocks have changed greatly, but the apoptosis rate compared with the ground control group no significant difference.