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目的研究尾部悬吊对大鼠比目鱼肌肌型和梭内外肌纤维胆碱酯酶活性的影响 ,探讨失重或模拟失重状态下肌肉萎缩和肌型转换的机制。方法选用雌性大鼠 ,以尾部悬吊法模拟失重。实验分为尾部悬吊7d、1 4d、2 1d组及相应的同步饲养对照组。以琥珀酸脱氢酶 (SDH)确定肌型 ,检测不同类型梭内外肌纤维乙酰胆碱酯酶 (AChE)活性。结果悬吊使肌肉萎缩、梭外肌纤维构成比发生改变 ,Ⅰ型纤维减少而Ⅱ型纤维增多。Ⅰ、Ⅱ型梭外肌纤维和梭内肌纤维乙酰胆碱酯酶活性均减弱。结论模拟失重可导致神经 -肌接头胆碱酯酶活性减弱。这种变化可能与运动神经元活动下降有关
Objective To investigate the effect of tail suspension on the activity of muscle fibers and muscle fibers in and out of the rat soleus muscle and to explore the mechanism of muscle atrophy and muscle conversion under conditions of weightlessness or simulated weightlessness. Methods Female rats were selected to simulate weightlessness by tail suspension method. The experiment was divided into tail suspension 7d, 14d, 21d group and the corresponding synchronous feeding control group. The muscle type was determined by SDH, and AChE activities of different types of intra-and extra-myofibrillar fibers were detected. Results Suspension caused muscular atrophy, the composition ratio of extrafusal muscle fibers changed, type Ⅰ fibers decreased and type Ⅱ fibers increased. The activity of acetylcholinesterase of type I and type II extrasolar muscle fibers and intrafusal myofibers were weakened. Conclusion Simulated weightlessness can lead to the decrease of cholinesterase activity in nerve-muscle connector. This change may be related to decreased motor neuron activity