多杀霉素是一种新型绿色广谱生物杀虫剂,属于微生物源生化农药,在进行了急性毒性和致突变试验的基础上,为了进一步明确受试样品91%多杀霉素原药的毒性,取得该样品亚慢性经口毒性的最大无作用剂量参数,依据GB 15670-1995《农药登记毒理学试验方法》[1]对其进行大鼠亚慢性经口毒性试验。1材料与方法1.1材料1.1.1受试物:91%多杀霉素原药,主要成分为多杀霉素,含量91%,性状为灰白色粉末固体。1.1.2实验动物和饲养环境:健康SPF级SD大鼠88 Spiramycin is a new type of green broad-spectrum biological insecticide, which belongs to microbial biochemical pesticides. On the basis of acute toxicity and mutagenicity test, in order to further clarify the 91% spinosad original drug The maximum non-effective dose parameters for sub-chronic oral toxicity of the sample were obtained. The subchronic oral toxicity test of rats was conducted according to GB 15670-1995 “Toxicological test of pesticide registration” [1]. 1 Materials and methods 1.1 Materials 1.1.1 Test substance: 91% spinosad original drug, the main component of spinosad, content of 91%, traits as an off-white powdery solid. 1.1.2 Experimental animals and feeding environment: healthy SPF SD rats 88