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目的 考察雌酚酮对骨组织c fos和c jun表达的影响 ,为研究雌激素对骨的作用机制积累资料。方法 用体外培养胎鼠长骨考察了雌酚酮对骨生长的作用 ,并采用免疫组织化学方法测定了其对骺板静止区、增殖区和肥大区c fos和c jun蛋白质表达的影响。结果 雌酚酮在体外能促进长骨生长 ,并在 10 -9mol·L-1～ 10 -7mol·L-1浓度范围内以剂量依赖的方式上调 3个区c fos和c jun蛋白质表达水平。结论 提示雌酚酮可能通过上调c fos和c jun在软骨细胞的表达而促进含有AP 1结合位点的、与骨生长发育有关的基因转录和表达 ,从而促进骨生长
Objective To investigate the effect of estrone on the expression of c fos and c jun in bone tissue and to accumulate data on the mechanism of action of estrogen on bone. Methods The effect of estrone on the growth of bone was investigated in vitro. The effects of estrone on the protein expression of c fos and c jun in static zone, proliferative zone and hypertrophic zone of epiphyseal plate were measured by immunohistochemistry. Results The estrone could promote the growth of long bones in vitro and up-regulate the protein expression levels of c fos and c jun in a dose-dependent manner in the concentration range of 10 -9 mol·L -1 -10 -7 mol·L -1. Conclusions The results suggest that estrone may promote the growth and development of osteoblasts by up-regulating the expression of c fos and c jun in chondrocytes and promoting the transcription and expression of genes involved in bone growth and development, including AP 1 binding sites