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了解辽宁省1997~2014年流行麻疹野病毒血凝素(Hemagglutinin,H)基因特征,为控制和消除麻疹提供依据。本研究选用1997~2014年分离的63株H1a基因亚型麻疹病毒(Measles,MEV),应用逆转录-聚合酶链反应(RT-PCR)分别扩增麻疹病毒(MEV)血凝素(H)基因全长和N基因的450个核苷酸片段,对扩增产物进行核苷酸序列测定和分析。将辽宁省63株MEV序列与GenBank中收录的1993~1994年麻疹病毒H基因型代表株、中国疫苗株沪191(S-191)和长春47(C-47)基于H全长构建基因亲缘性关系树,进行核苷酸和氨基酸变异分析。结果显示辽宁省1997~2014年63株MEV均属于H1基因型的H1a亚型,并分为两个簇(cluster 1和2)。H基因的进化速度低于N基因的进化速度。所有63株MeV在H基因第240位均由丝氨酸(Ser S)突变为天冬酰胺酸(Asn N),在243位由精氨酸(Arg R)突变成甘氨酸(Gly G),第481位由酪氨酸(Tyr Y)突变为天冬酰胺酸(Asn N),另外在397位上cluster 2毒株均由脯氨酸(Pro P)突变成亮氨酸(Leu L),其他具有生物学和免疫学活性的位点未发生改变。
To understand the characteristics of Hemagglutinin (H) gene of wild measles virus from 1997 to 2014 in Liaoning Province, and provide evidence for controlling and eliminating measles. In this study, 63 strains of H1 measles (MEV) isolated from 1997 to 2014 were used to amplify measles virus (MEV) hemagglutinin (H) by reverse transcription-polymerase chain reaction (RT- Length of the gene and 450 nucleotides of the N gene, the amplified products were subjected to nucleotide sequence determination and analysis. Sixty-three MEV sequences of Liaoning Province were compared with those of the representative H genotypes of measles virus from 1993 to 1994, the Chinese vaccine strains Shanghai 191 (S-191) and Changchun 47 (C-47) Relationship tree for nucleotide and amino acid variation analysis. The results showed that all the 63 MEV isolates in Liaoning province from 1997 to 2014 belonged to H1 subtype of H1 genotype and were divided into two clusters (cluster 1 and 2). The rate of evolution of the H gene is lower than that of the N gene. All 63 MeVs were mutated from serine to asparagine at position 240 of H gene, from Arg R to Gly G at position 243, (Tyr Y) to Asn N, and the cluster 2 strain was mutated from Pro P to Leu L at position 397. The other Sites with biological and immunological activity did not change.