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目的探讨乙型肝炎病毒(HBV)对胶原三股螺旋重复蛋白1(CTHRC1)表达的影响及其调节的分子机制。方法将HBV感染性克隆p HBV1.3及其单个基因的质粒分别与CTHRC1基因启动子共转染Hep G2细胞,并测定荧光素酶的活性;采用反转录PCR和Western blot检测CTHRC1 mRNA和蛋白表达的情况。结果 HBV X蛋白能够在Hep G2细胞显著激活CTHRC1基因启动子的活性,并在mRNA和蛋白水平上调CTHRC1的表达。结论 HBV通过X蛋白上调CTHRC1的表达。
Objective To investigate the effect of hepatitis B virus (HBV) on the expression of CTHRC1 and its molecular mechanism. Methods The HBV infectious clone p HBV1.3 and its single gene plasmids were cotransfected into Hep G2 cells with CTHRC1 gene promoter respectively and the activity of luciferase was determined. Reverse transcription PCR and Western blot were used to detect CTHRC1 mRNA and protein Express the situation. Results HBV X protein could significantly activate the promoter of CTHRC1 gene in Hep G2 cells and up-regulate the expression of CTHRC1 at mRNA and protein level. Conclusion HBV can up-regulate the expression of CTHRC1 through X protein.