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目的研究共轭亚油酸(CLA)对高脂高糖饮食诱导的肥胖大鼠体质量、血脂及肝脏脂质代谢的影响。方法成年雌性Sprague-Dawley大鼠35只按体质量随机分为5组,即基础对照组、肥胖对照组和CLA低、中、高剂量组(分别为0.4、0.81、.6 g.kg-1)。除基础对照组喂饲基础饲料外,其余4组喂饲高脂高糖饲料,喂饲5周后灌胃CLA 1个月,测定体质量和血脂水平,并对肝脏组织切片进行油红O染色。结果与肥胖对照组相比,CLA低、中、高剂量组体质量分别降低了5.28%、5.67%、7.16%,CLA 3个剂量组的脂体比分别降低了52.8%、54.6%、54.6%(P<0.05);CLA 3个剂量组的血清甘油三酯水平分别降低了18.6%、34.8%、49.1%,其中高剂量组与肥胖对照组相比差异有显著性(P<0.05);CLA组大鼠肝脏切片油红O染色后肝细胞内的红染脂滴随CLA剂量的增加逐渐减少。结论CLA可降低大鼠体质量、血脂水平,并通过影响肝脏脂质代谢起到抗肥胖的作用。
Objective To investigate the effects of conjugated linoleic acid (CLA) on body weight, lipids and lipid metabolism in the liver of obese rats induced by high fat and sucrose diet. Methods Thirty-five adult female Sprague-Dawley rats were randomly divided into five groups according to body weight: basic control group, obesity control group and CLA low, medium and high dose groups (0.4, 0.81, .6 g.kg -1 ). In addition to the basic control group fed basal diet, the other four groups were fed with high-fat and high-sucrose diet, fed with CLA for 1 month after 5 weeks of feeding, body weight and blood lipid levels were measured, and liver tissue sections were subjected to oil red O staining . Results Compared with the obese control group, the body weight of CLA low, middle and high dose groups decreased by 5.28%, 5.67% and 7.16% respectively, and the lipid ratio of CLA 3 dose groups decreased by 52.8%, 54.6% and 54.6% (P <0.05). The level of serum triglyceride in CLA 3 dose group decreased by 18.6%, 34.8% and 49.1%, respectively, and there was significant difference between high dose group and obesity control group (P <0.05) The red dye droplets in hepatocytes of the rats’ liver slices were decreased with the increase of CLA dosage. Conclusion CLA can reduce body weight and blood lipid levels in rats and play an anti-obesity role by affecting lipid metabolism in the liver.