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[目的]优化仁用杏微卫星非变性聚丙烯酰胺凝胶电泳体系。[方法]以25份仁用杏和3份食用杏为材料,利用微卫星标记结合非变性聚丙烯酰胺凝胶电泳技术筛选出多态性高、可重复的SSR位点。并比较不同因子对电泳体系的影响,探索仁用杏微卫星非变性聚丙烯酰胺凝胶电泳的优化体系。[结果]仁用杏微卫星非变性聚丙烯酰胺凝胶电泳的优化体系为:聚丙烯酰胺凝胶的浓度为6%,丙烯酰胺与甲叉双丙烯酰胺的比例为29∶1,1000V下电泳2～3h,0.1%AgNO3染色15min后显影。[结论]该优化体系为仁用杏资源微卫星标记遗传分析奠定技术基础。
[Objective] The research aimed to optimize the apricot microsatellite non-denaturing polyacrylamide gel electrophoresis system. [Method] With 25 apricots and 3 apricots as materials, SSR loci with high polymorphism and repeatability were screened by microsatellite markers combined with native polyacrylamide gel electrophoresis. The influence of different factors on the electrophoresis system was compared, and the optimized system of aptamer microsatellite non-denaturing polyacrylamide gel electrophoresis was explored. [Result] The optimized system of apricot microspore non-denaturing polyacrylamide gel electrophoresis was as follows: the concentration of polyacrylamide gel was 6%, the ratio of acrylamide to methylene bisacrylamide was 29:1, the electrophoresis was at 1000V 2 ~ 3h, 0.1% AgNO3 staining after 15min development. [Conclusion] The optimized system laid the technical foundation for genetic analysis of microsatellite marker of apricot resources.