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目的建立测定参茸珍宝片中特女贞苷含量的方法。方法采用反相高效液相色谱法,色谱柱为Ultimate XB-C18-H(250 mm×4.6 mm,5-m),流动相为甲醇-水(38∶62),流速为1.0 mL·min-1,检测波长为224 nm,进样量为10μL。结果特女贞苷进样量在0.053 44~1.603 3-g内与峰面积积分值线性关系良好(r=0.999 9);平均加样回收率为96.90%,RSD为0.93%。结论该方法快速简便、准确可靠、重复性好,可用于参茸珍宝片中特女贞苷的含量测定。
Objective To establish a method for the determination of the telmisartan in ginseng treasure tablets. Methods The RP-HPLC method was used. The column was Ultimate XB-C18-H (250 mm × 4.6 mm, 5-m) and the mobile phase consisted of methanol-water (38:62). The flow rate was 1.0 mL · min- 1, detection wavelength of 224 nm, injection volume of 10μL. The results showed that the linearity of the peak value was significant (r = 0.999 9) in the 0.053 44 ~ 1.603 3-g injection with the average recovery of 96.90% and RSD 0.93%. Conclusion The method is rapid, simple, accurate, reliable and reproducible. It can be used to determine the content of lignoside in ginseng jelly tablets.