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目的:观察损伤对成年动物骨骼肌卫星细胞增殖的影响,以获取基因治疗自体移植的载体。方法:以阳离子脂质体介导法将ｐＲＳＶＬａｘＺ或ｐＲｃｈＴＨ质粒转入培养肌细胞。结果:骨骼肌损伤后,分离出的肌卫星细胞及组织块游离成肌细胞数均显著增高;外源基因能在细胞内表达。结论:损
OBJECTIVE: To observe the effect of injury on the proliferation of adult animal skeletal muscle satellite cells in order to obtain gene therapy autologous transplantation vector. Methods: Plasmid pRSVLaxZ or pRchTH was transfected into myoblasts by cationic liposome-mediated method. RESULTS: After skeletal muscle injury, the numbers of free myoblasts of muscle satellite cells and tissue blocks were significantly increased. The exogenous genes could be expressed in the cells. Conclusion: Loss