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用视网膜电位图 (electroretinogram ,ERG)技术研究了棉铃虫Helicoverpaarmigera蛾暗适应过程中对单色光和白光刺激的光感受性变化。结果显示 :(1)依ERG振幅大小 (峰 峰值 ) ,在 34 0～ 6 0 5nm波谱内有 3个大小不等的峰———主峰位于绿 黄光区 5 6 2nm ,次峰在蓝光区 483nm ,第 3个峰在近紫外区 40 0nm ,显示其至少有 3种感受器 ;(2 )性别、日龄及暗适应时间长短对其光谱敏感性有影响 ,低龄时雄蛾对单色光刺激较雌蛾敏感 ,高日龄时相反 ;1～ 5日龄内 ,3日龄蛾的视网膜电位 (ERP)值最高 ;随暗适应时间延长 ,其复眼对近紫外区 40 0nm敏感性明显增加 ;(3)一定光强度范围内 ,随单色光和白光光强度增强该蛾复眼的ERP值增大 ,初期增加较缓 ,中期较快 ,呈近似S型曲线 ,显示其复眼具有较强的光强度自调节和适应机制。
The electroretinogram (electroretinogram, ERG) technique was used to study the changes of photoreceptors of monochromatic light and white light during dark adaptation of Helicoverpa armigera. The results showed that: (1) According to the amplitude of ERG (peak-to-peak value), there are three peaks of different sizes in the range of 34 0-6 500 nm --- the main peak is located at 562 nm in the green and yellow region, 483nm, the third peak in the near-ultraviolet region at 40 0nm, showing at least 3 receptors; (2) sex, age and dark adaptation of the length of the spectral sensitivity of its influence, young male monk light stimulation Compared with female moths, the values of ERP in the 3rd instar larvae were the highest at 1 to 5 days old. The sensitivity of compound eye to 400 nm in the near ultraviolet region was significantly increased with dark adaptation time. (3) ) Within a certain range of light intensity, with the monochromatic light and white light intensity increased the moth compound eyes ERP value increases, the initial increase more slowly, the mid-fast, was approximately S-shaped curve, showing that the compound eye has a strong light intensity since Regulation and adaptation mechanism.