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采用移植型小鼠黑色素瘤B_(16)模型进行试验,研究灰树花菌丝体糖肽GFPS1b的体内抗肿瘤作用。结果表明,灰树花菌丝体糖肽GFPS1b对移植型小鼠黑色素瘤B_(16)有显著的抑制作用,抑瘤率在30%以上;样品作用组瘤体积生长速率比阴性对照组慢,且瘤体积小;同时能延长荷瘤小鼠的存活期;HE染色结果表明,GFPS1b中、高剂量组能使B16肿瘤细胞呈团索状实性排列或弥漫性分布,并有肿瘤细胞大片坏死,坏死区周围可见凋亡细胞;免疫组化技术进一步显示,GFPS1b能诱导瘤组织中Bax基因蛋白表达,同时也能显著下调Bcl-2蛋白的表达。研究表明,GFPS1b对小鼠移植性肿瘤有显著的抑瘤作用。
The transplanted mouse melanoma B_ (16) model was used to study the anti-tumor effect of Grifola frondosa glycopeptide GFPS1b in vivo. The results showed that Grifola frugiperda glycopeptide GFPS1b had a significant inhibitory effect on the transplanted mouse melanoma B_ (16) and the tumor inhibition rate was above 30%. The tumor volume growth rate of the sample was slower than that of the negative control group, And tumor size is small; at the same time can prolong the survival of tumor-bearing mice; HE staining results show that, GFPS1b medium and high dose group can make B16 tumor cells were organized cable-like arrangement or diffuse distribution, and tumor necrosis , And apoptotic cells were observed around the necrotic area. Immunohistochemistry further showed that GFPS1b could induce the expression of Bax gene in tumor tissue, and also significantly down-regulate the expression of Bcl-2 protein. Studies have shown that, GFPS1b on mice transplanted tumor significant anti-tumor effect.