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树突状细胞(Dendritic cell,DC)在肿瘤主动特异性免疫治疗中起重要作用,肿瘤抗原冲激的DC作为一种新型瘤苗颇受研究者的重视.本实验中我们选择小鼠肝癌H22的高淋巴道转移亚系HCa-F(H-2~-)为主要肿瘤模型,采用榄香烯瘤苗可溶性上清冲激小鼠脾脏DC,研究抗原冲激的DC对HCa-F的免疫保护作用及机制.主要实验方法和结果如下:①从未处理的HCa-F细胞及其榄香烯瘤苗中提取可溶性上清冲激正常小鼠脾脏DC,所得DC分别称为HCa-F上清冲激的DC和瘤苗上清冲激的DC(以下简称HDC和TDC).②转输HDC或TDC(5×10~5个细胞/只)给正常615系小鼠,7d后用HCa-F(5×10~5个细胞/只)攻击,观察小鼠瘤重及存活时
Dendritic cells (DCs) play an important role in tumor-specific active immunotherapy. Tumor antigen-pulsed DCs have attracted much attention as researchers. In this experiment we selected mouse liver cancer H22. The high-lymphoid metastasis subfamily HCa-F (H-2~-) was the main tumor model. The soluble supernatant of elemene vaccine was used to excite the mouse spleen DC to study the immune response of antigen-pulsed DC to HCa-F. Protective effects and mechanisms. The main experimental methods and results are as follows: 1 Extracting soluble supernatants from untreated HCa-F cells and their elemene neoplasms stimulates spleen DC of normal mice. The resulting DCs are called HCa-F, respectively. DCs with clear impulses and DCs pulsed with tumor supernatants (HDCs and TDCs).2 DCs transfused with HDCs or TDCs (5 x 10~5 cells/cells) to normal 615 lineage mice, 7 days later with HCa -F (5 x 10~5 cells/body) challenge to observe tumor weight and survival in mice