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目的 : 阐明克山病心肌细胞损害的电生理机制。方法 : 用细胞内微电极技术分别观察正常对照、单纯低 Se、低 Se高维生素 C、单纯低 Cr、低 Cr高维生素 C、低 Se低 Cr、低 Se低Cr伴高维生素 C饲料饲养 1 4w后大鼠心肌细胞电生理的改变。结果 : 单纯低 Cr组无变化 ;单纯低硒组静息电位 ( RP)、动作电位幅度 ( APA)降低、动作电位时程 ( APD50 、APD90 )延长 ;低 Se低Cr组 RP、APA降低 ,APD50 、APD90 明显延长 ;低 Se高维生素 C组仅 RP降低 ,APA、APD与对照组相比差异无显著性意义。结论 : 低 Se低 Cr可明显改变心肌细胞的电生理特性 ,维生素 C可以对抗低 Se低 Cr所致的这种变化。
Objective: To elucidate the electrophysiological mechanism of Keshan disease in cardiomyocytes. Methods: The normal control, low Se, low Se high vitamin C, simple low Cr, low Cr high vitamin C, low Se low Cr, low Se low Cr with high vitamin C diet were observed by intracellular microelectrode technique. Electrophysiological Changes of Rat Cardiomyocytes. RESULTS: The resting potential (RP), APA (APA) and the action potential duration (APD50, APD90) in pure low selenium group were prolonged. The RP and APA , APD90 significantly prolonged; low Se high vitamin C group only reduced RP, APA, APD compared with the control group no significant difference. CONCLUSION: Low Se and low Cr significantly change the electrophysiological characteristics of cardiomyocytes. Vitamin C can resist this change caused by low Cr and low Se.