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目的:动态观察肾间质纤维化大鼠肾组织中结缔组织生长因子(CTGF)蛋白的表达变化以及益肾化瘀方对其影响,探讨益肾化瘀方治疗肾间质纤维化的部分机制。方法:将150只大鼠随机分为假手术组、模型组、益肾化瘀方高剂量组、益肾化瘀方低剂量组、洛汀新组,采用单侧输尿管结扎(UUO)的方法建立肾间质纤维化大鼠模型;分别于UUO术后3、7、14、21、28d观察模型大鼠肾组织中CTGF蛋白的表达变化,采用Western-blot方法,以β-actin为内参,测定CTGF蛋白的相对表达量。结果:与假手术组相比,模型组大鼠肾组织CTGF蛋白表达于7d后出现不同程度的增高(P<0.01);用药后至第7d,与模型组相比,各给药组大鼠肾组织CTGF蛋白表达均有所降低(P<0.05或P<0.01),益肾化瘀方高剂量组优于益肾化瘀方低剂量组和洛汀新组(P<0.05或P<0.01)。结论益肾化瘀方可以通过降低肾组织中CTGF蛋白表达,从而防治肾间质纤维化。
OBJECTIVE: To dynamically observe the expression of connective tissue growth factor (CTGF) in renal tissue of rats with renal interstitial fibrosis and the effect of Yishen Huayu Recipe on it, and to explore the mechanism of Yishen Huayu Recipe in treating renal interstitial fibrosis . Methods: A total of 150 rats were randomly divided into sham operation group, model group, Yishen Huayu Recipe high dose group, Yishen Huayu Recipe low dose group and Lotensin group. Unilateral ureteral ligation (UUO) The rat model of renal interstitial fibrosis was established. The changes of CTGF protein expression in the kidney of model rats were observed at 3, 7, 14, 21 and 28 days after UUO. Western-blot and β-actin were used as internal reference, The relative expression of CTGF protein was determined. Results: Compared with sham operation group, the expression of CTGF protein in renal tissue of rats in model group increased to some extent after 7 days (P <0.01). Compared with model group, (P <0.05 or P <0.01). The Yishen Huayu Recipe high dose group was better than Yishen Huayu Recipe low dose group and Lotensin group (P <0.05 or P <0.01) ). Conclusion Yishen Huayu Recipe can prevent and cure renal interstitial fibrosis by reducing CTGF protein expression in renal tissue.