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目的:考察小叶丁香总皂苷对D-半乳糖致衰老模型小鼠抗氧化作用。方法:采用小鼠颈背部皮下注射D-半乳糖溶液200 mg·kg-1·d-1建立衰老模型;造模成功后,将小鼠随机分为模型对照组、小叶丁香总皂苷低剂量组(60 mg·kg-1)、小叶丁香总皂苷高剂量组(120 mg·kg-1)和阳性药对照组(维生素E,50 mg·kg-1),连续给药8周后检测血及脑组织中的超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)和总抗氧化能力(T-AOC)等指标。结果:与模型对照组比较,小叶丁香总皂苷低剂量组能明显升高血清中的SOD、GSH-Px和CAT活性以及脑组织中的T-AOC活性;小叶丁香高剂量组能显著降低小鼠血清及脑组织中的MDA水平,升高小鼠血清中的SOD、GSH-Px、CAT和T-AOC活性以及脑组织中的SOD、GSH-Px和T-AOC活性。结论:小叶丁香总皂苷能抑制机体内脂质过氧化物的生成,增强抗氧化酶的活性,从而提高衰老小鼠的抗氧化能力,延缓衰老。
OBJECTIVE: To investigate the anti-oxidative effect of clover saponin on D-galactose induced aging model mice. Methods: Aging models were established by subcutaneous injection of D-galactose solution 200 mg · kg-1 · d-1 into the back of the neck of mice. After successful modeling, the mice were randomly divided into model control group, (60 mg · kg-1), high-dose total clover saponins (120 mg · kg-1) and positive control group (vitamin E, 50 mg · kg-1) The levels of superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), catalase (CAT) and total antioxidant capacity (T-AOC ) And other indicators. Results: Compared with the model control group, the low dose of Clove Saponins could significantly increase the activities of SOD, GSH-Px and CAT in serum and the activity of T-AOC in brain tissue; Serum and brain MDA levels increased the activities of SOD, GSH-Px, CAT and T-AOC in serum and SOD, GSH-Px and T-AOC activities in brain tissue. Conclusion: Clove Scutellariae total saponin can inhibit the formation of lipid peroxides in the body and enhance the activity of antioxidant enzymes, thereby enhancing the antioxidant capacity of aging mice, anti-aging.