论文部分内容阅读
目的观察槟榔水提取物对骨质疏松小鼠骨密度及氧化应激状态的影响,并初步探讨其机制。方法将24只KM小鼠随机均分为对照组、骨质疏松组和槟榔组共3组,每组8只。骨质疏松组和槟榔组通过切除卵巢建立骨质疏松模型,对照组不建立骨质疏松模型;术后,槟榔组在饮用水中添加槟榔浸出液,对照组和骨质疏松组饮用水中不添加槟榔浸出液。6个月后测量小鼠体重及骨密度,检测小鼠血液中过氧化氢(H2O2)、丙二醛(MDA)、谷胱甘肽(GSH)、过氧化氢酶(CAT)的含量,及骨保护素(OPG)和核因子κB受体活化因子配体(RANKL)含量。结果术后6个月,与骨质疏松组比较,槟榔组体重增加(P<0.05),骨密度升高(P<0.05),H2O2和MDA含量下降(P<0.05),GSH含量升高(P<0.05)。骨质疏松组CAT含量较槟榔组下降(P<0.05),而槟榔组CAT含量与对照组比较差异无统计学意义(P>0.05)。结论槟榔可以有效抑制小鼠骨质疏松的进展,其机制可能与其有效抑制OPG的降低和RANKL的升高,及有效抑制氧化应激状态有关。
Objective To observe the effects of betel nut water extract on bone mineral density (BMD) and oxidative stress in osteoporotic mice and to explore its mechanism. Methods 24 KM mice were randomly divided into control group, osteoporosis group and betel nut group, a total of 3 groups, 8 in each group. Osteoporosis group and betel nut group by ovariectomy to establish osteoporosis model, the control group does not establish osteoporosis model; after betel nut group added betel nut extract in drinking water, the control group and the osteoporosis group drinking water is not added Betel nut leachate. After 6 months, the body weight and bone mineral density of the mice were measured. The content of H2O2, MDA, GSH and CAT in the blood of the mice were measured, and Osteoprotegerin (OPG) and nuclear factor kappa B receptor activator ligand (RANKL) content. Results Compared with the osteoporosis group, body weight increased (P <0.05), bone mineral density increased (P <0.05), H2O2 and MDA content decreased (P <0.05) and GSH content increased P <0.05). The content of CAT in osteoporosis group was lower than that in areca group (P <0.05), while CAT content in areca nut group was not significantly different from that in control group (P> 0.05). Conclusion Betel nut can effectively inhibit the progress of osteoporosis in mice. The mechanism may be related to the effective inhibition of the decrease of OPG and the increase of RANKL and the inhibition of oxidative stress.