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目的研究生存素mRNA在膀胱移行细胞癌(TCC)中的表达,及其与TCC的病理分级、临床分期的相关性。方法用半定量逆转录聚合酶链反应法对30例TCC患者的肿瘤组织、3例良性前列腺增生症(BPH)和2例外伤所致膀胱破裂患者的膀胱组织进行生存素mRNA检测。结果30例TCC组织生存素mRNA表达全部呈阳性,而3例BPH患者和2例膀胱破裂患者均呈阴性。TCCⅠ级、Ⅱ级、Ⅲ级患者生存素mRNA与肌动蛋白mRNA灰度比值分别为0·95±0·12,1·25±0·16,1·52±0·10,组间差异显著(P<0·01);TCC临床分期未突破基底膜、侵及浅肌层、侵及深肌层的患者生存素mRNA、与b-MGmRNA灰度比值分别为1·00±0·26,1·23±0·16,1·47±0·17,组间差异均显著(P<0·05)。结论膀胱粘膜生存素mRNA的表达可用于TCC患者的辅助诊断及恶性程度和预后的判断。
Objective To study the expression of survivin mRNA in bladder transitional cell carcinoma (TCC) and its relationship with pathological grade and clinical stage of TCC. Methods Survivin mRNA was detected by semiquantitative reverse transcription polymerase chain reaction (RT-PCR) in 30 TCC patients with tumor tissue, 3 benign prostatic hyperplasia (BPH) and 2 bladder cancer patients with bladder rupture. Results The survivin mRNA expression was positive in all 30 TCC tissues, while 3 BPH patients and 2 bladder rupture patients were negative. The ratios of survivin mRNA to actin mRNA in patients with TCC grade Ⅰ, Ⅱ and Ⅲ were 0.95 ± 0.12, 1.25 ± 0.16 and 1.52 ± 0.10, respectively, with significant difference between the two groups (P <0.01). Survivin mRNA and b-MG mRNA gray scale in patients with TCC without clinical stage involvement in basal lamina, invasion of superficial myometrium and deep muscularis were 100.00 ± 0.226, 1 · 23 ± 0 · 16,1 · 47 ± 0 · 17, there was significant difference between the two groups (P <0.05). Conclusions The expression of survivin mRNA in bladder mucosa can be used to diagnose and evaluate the degree of malignancy and prognosis of patients with TCC.