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目的体外培养糖尿病与非糖尿病冠心病患者的骨髓间充质干细胞(mesenchymal stem cells,MSCs),采用Affymetrix基因芯片技术对糖尿病与非糖尿病冠心病患者的MSCs功能基因表达谱进行比较。方法冠心病合并糖尿病组纳入1例患者,男,53岁;诊断:冠心病、2型糖尿病。非糖尿病冠心病组纳入1例患者,男,51岁;诊断:冠心病。将两组患者的骨髓淋巴细胞分离液采用密度梯度离心法和贴壁法进行分离、提纯MSCs,再用Affymetrix全基因组芯片检测MSCs特异性功能蛋白基因表达的差异。结果冠心病合并糖尿病组功能蛋白表达基因中涉及细胞凋亡、细胞因子、细胞内信号传导系统的基因有27个,其中13个基因表达明显上调,分别为TNFRSF10B、TNFRSF21、NGF、CAV2、ITGA8、TNS1、ITGA2、AKT3、MBP、MAP2、INHBA、FST、PLA2G5;有14个基因表达明显下调,分别为EPR1、BIRC5、HELLS、BCL2、HGF、CASP1、SEPP1、ITGA9、MAP2K6、RUNX3、TGFBR2、RUNX2、CTNNB1、CDC42。结论糖尿病合并冠心病患者的MSCs在功能基因表达方面存在显著变化。
OBJECTIVE: To culture mesenchymal stem cells (MSCs) in diabetic and non-diabetic patients with coronary heart disease and to compare the functional gene expression profiles of MSCs in diabetic and non-diabetic patients with coronary heart disease using Affymetrix microarray. Methods One patient with coronary heart disease complicated with diabetes mellitus was included, male, 53 years old; diagnosis: coronary heart disease, type 2 diabetes mellitus. One patient was enrolled in the non-diabetic coronary heart disease group, male, 51 years old; diagnosis: coronary heart disease. The bone marrow lymphocytes from both groups were separated by density gradient centrifugation and adherent method to purify MSCs. The Affymetrix whole genome microarray was used to detect the difference of MSCs specific functional protein gene expression. Results There were 27 genes involved in apoptosis, cytokines and intracellular signal transduction in functional protein expression in CHD patients. Among them, 13 genes were upregulated, including TNFRSF10B, TNFRSF21, NGF, CAV2, ITGA8, 14 genes were downregulated, including EPR1, BIRC5, HELLS, BCL2, HGF, CASP1, SEPP1, ITGA9, MAP2K6, RUNX3, TGFBR2, RUNX2, CTNNB1, CDC42. Conclusion MSCs in diabetic patients with coronary heart disease have significant changes in functional gene expression.