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酪胺在~(125)I标记过程中生成单~(125)I和二~(125)I酪胺混合物,通常还有未起反应的酪胺,明显降低最终产物的比活性.闸述了从含有游离~(125)I和酪胺载体的混合物中分离单~(125)I和/或二~(125)I酪胺的方法,以获得高比活性~(125)I酪胺衍生物.酪胺的放射性碘标记过程:10μl酪胺(0.73mmol/L),10μl Na~(125)I(9.25TBq/L于0.25mol/L pH7.5PB中)和5μl氯胺-T(3.55mmol/L于0.25mol/L pH7.5PB中),室温(20～22℃)温育70秒钟;5μl偏重亚硫酸钠(5.26mmol/L于0.25mol/L pH7.5PB中)终止反应.碘化反应混合物比活性12.95TBq/mmol.
Tyramine produces a mixture of mono- (125) I and di- (125) I tyramine and ~ (125) I tyramine in the ~ (125) I labeling process, often with unreacted tyramine, which significantly reduces the specific activity of the final product. A method for separating mono- (125) I and / or di- (125) I tyramine from a mixture containing free ~ (125) I and a tyramine carrier is used to obtain a high specific activity ~ (125) I tyramine derivative Radioiodination of tyramine: 10 μl of tyramine (0.73 mmol / L), 10 μl of Na 125 I (9.25 TBq / L in 0.25 mol / L pH 7.5 PB) and 5 μl of chloramine-T / L in 0.25 mol / L pH7.5PB), incubated for 70 seconds at room temperature (20-22 C), and 5 l sodium metabisulfite (5.26 mmol / L in 0.25 mol / L pH 7.5PB) The specific activity of the mixture was 12.95TBq / mmol.