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目的：探寻新生儿重症监护室医务人员手消毒效果监测一种新的采样方法。方法：用浸有含相应中和剂的无菌洗脱液的4cm*5cm 无菌湿纱布平铺于医务人员手双手心并拢采样后按标准培养，培养100人次；同时用标准采样法采集100人次应用 Q 检验去除离群值。结果：医务人员手新方法采样100次培养菌落数2－8CFU ／cm2，平均3．8 CFU ／cm2。传统标准方法采样100次培养菌落数3－8 CFU ／cm2平均4．5 CFU ／cm2两组数据相似。结论：这种采样方法便捷，结果可靠。“,”To find a new sampling method in monitoring disinfection effect of hands of medical staff in NICU.Methods:Use germ free gause soaked with aseptic eluentsterile containing appropriate neutralizer (4cm*5cm),put in the hands of medical staff to sample,100 people cultured,after sampling culture according to the standard.At the same time,collect the same 100 staffs using standard sampling method.use Q inspection to remove outliers,Results:new method collecting 100 hands of medical staff cultured colony number vary from 2 -8CFU /cm2,average 3.8 CFU /cm2.100 cases using standard sampling method colony number vary from 3 -8CFU /cm2,average 4.5 CFU /cm2..The two groups data are similar.Conclusion:This sampling method is convenient, reliable .