论文部分内容阅读
目的研究海藻酸钠聚赖氨酸海藻酸钠包裹胰岛进行移植的效果。方法将Ｗｉｓｔａｒ大鼠的胰腺先行胶原酶胰管内注射消化，然后分离、纯化，所得胰岛经培养后制成微囊包膜的胰岛，微囊直径为０．４～０．５ｍｍ，每个微囊内包含１个胰岛。将微囊包膜及非包膜的胰岛移植至小鼠腹腔内，每个受体的移植量约１０００个胰岛。术后不用免疫抑制剂。结果微囊化的胰岛分别置含葡萄糖５．６ｍｍｏｌ／Ｌ和１６．７ｍｍｏｌ／Ｌ的无血清ＴＣ１９９培养基中培养，其胰岛素的释放量，包膜组与非包膜组相比，差异不显著（Ｐ＞０．０５）。微囊化胰岛移植组术后３天非空腹血糖从（２２．０±０．５１）ｍｍｏｌ／Ｌ降至（７．８±０．４８）ｍｍｏｌ／Ｌ，维持正常血糖的时间平均为１２６天；单纯胰岛移植组术后移植物有功能存活不足８天。结论微囊化胰岛移植后可在不使用免疫抑制剂的情况下延长胰岛移植物的存活时间，逆转高血糖
Objective To study the effect of sodium alginate-polylysine-sodium alginate encapsulated islet transplantation. Methods Wistar rat pancreatic progenitor collagenase pancreatic duct injections, digestion, and then isolated and purified, the resulting islets were cultured into microcapsule-coated islets, the microcapsule diameter of 0.4 ~ 0.5mm, each microcapsule Contains 1 islet. The microencapsulated and non-enveloped pancreatic islets were transplanted into the peritoneal cavity of mice, and the transplanted amount of each is about 1000 islets. Postoperative immunosuppressive agents. Results Microencapsulated islets were cultured in serum-free TC199 medium containing 5.6 mmol / L glucose and 16.7 mmol / L glucose respectively. There was no significant difference in insulin release between the encapsulated group and non-encapsulated group (P> 0.05). The non-fasting blood glucose decreased from (22.0 ± 0.51) mmol / L to (7.8 ± 0.48) mmol / L at 3 days after operation in the microencapsulated islet transplantation group, and the mean time to maintain normal blood glucose was 126 days ; Simple islet transplantation group postoperative graft survival less than 8 days. Conclusion Microencapsulated islet transplantation can prolong islet graft survival without immunosuppressive agents and reverse hyperglycemia