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[目的]获得中药三七、茯苓中克百威和抗蚜威的检测方法。[方法]通过液相色谱法对中药三七和茯苓中的克百威和抗蚜威的检测方法进行研究。[结果]确定了较优液相色谱检测条件为柱温30℃,流速1 mL/min,检测波长210 nm,流动相水-乙腈(体积比61.5∶38.5)。获得抗蚜威和克百威分别在1.99×10-4~9.95×10-4μg(r=0.9987)和1.97×10-4~9.85×10-4μg(r=0.9947)范围内线性良好。克百威和抗蚜威的RSD值分别为13.2%和7.1%。[结论]获得了克百威和抗蚜威的检测方法,并且2种中药样品中不含有克百威和抗蚜威。该方法出峰快,能避开受试中药中基质的干扰。
[Objective] To obtain the detection methods of Panax notoginseng, Poria coccicum and pirimicarb. [Method] The detection methods of carbofuran and pirimicarb in Panax notoginseng and tuckahoe were studied by liquid chromatography. [Result] The optimal HPLC conditions were as follows: column temperature 30 ℃, flow rate 1 mL / min, detection wavelength 210 nm, mobile phase water - acetonitrile (volume ratio 61.5:38.5). The obtained pirimicarb and carbofuran had good linearity in the range of 1.99 × 10-4 ~ 9.95 × 10-4μg (r = 0.9987) and 1.97 × 10-4 ~ 9.85 × 10-4μg (r = 0.9947) respectively. The RSD values for carbofuran and pirimicarb were 13.2% and 7.1%, respectively. [Conclusion] The detection methods of carbofuran and pirimicarb were obtained, and neither of the two traditional Chinese medicines samples contained carbofuran and pirimicarb. The method peak out quickly, to avoid the interference of the matrix in the test of Chinese medicine.