Extensive worldwide use of oxytetracycline(OTC), a member of the tetracyclines, has resulted in its accumulation in soils, posing a potential risk to food production and safety. A pair of OTC-sensitive(Heyou 1)and OTC-tolerant(Yannong 21)wheat(Triticum aestivum L.)cultivars was compared hydroponically at 0.01, 0.02, 0.04, and 0.08 mmol L(-1) OTC in terms of wheat growth and photosynthesis. Biomass and shoot length decreased significantly with the addition of OTC, with the decreases in dry biomass and shoot length being 5.61%-13.75% and 3.33%a-8.57% larger, respectively, for Heyou 1 than Yannong 21. Photosynthesis of Heyou 1,suppressed by OTC as indicated by the significant decreases in photosynthetic rate(PN), transpiration rate(TR), and stomatal conductance(GS)and the significant increase in intercellular CO2 concentrations(CI), at all OTC levels. Stomata] limitation(LS)and water use efficiencies(WUE)of Heyou 1 also increased significantly,but not at 0.08 mmol L(-1) OTC. However, photosynthesis of Yannong 21 was suppressed by OTC only at high OTC levels from 0.02 to 0.08 mmol L(-1) as indicated by the decreases in PN, GS,TR, and LS;at 0.01 mmol L(-1) OTC, PN, CI, GS, and TR significantly increased. It was noted that WUE of Yannong 21 was not affected by OTC addition. The results from this hydroponic test suggested that OTC had a potential risk to crop growth through inhibition of photosynthesis, requiring further confirmation with soil tests.