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目的探讨外源性一氧化氮(NO)在顺铂(DDP)作用于CNE-2细胞的过程中是否存在增敏效应,为提高鼻咽癌化疗疗效提供实验和理论依据。方法分别使用不同浓度NO供体药物硝普钠(SNP)、DDP及二者联合干预CNE-2细胞,采用硝酸还原酶法检测NO的浓度,镜下观察细胞形态学变化,CCK-8法检测细胞增殖抑制率以及流式细胞仪检测细胞凋亡。结果 (1)SNP呈浓度依赖方式抑制CNE-2细胞的增殖、促进凋亡;(2)上清液中NO浓度与SNP浓度呈正相关,有统计学意义(r=0.968,P<0.001);(3)DDP+SNP联合组与SNP组和DDP组相比,CNE-2细胞形态学差异显著,漂浮细胞显著增多,贴壁数量逐渐减少并失去原有形态;(4)当SNP 600μmol/L时对细胞增殖无明显影响,然而联合DDP后较单用DDP组细胞抑制率显著提高(t=9.049,P<0.001);(5)SNP组与DDP联用时CNE-2细胞凋亡率上升,较单用SNP、DDP组显著增强(t=-19.816,P<0.001;t=-5.242,P=0.035)。结论外源性NO能抑制CNE-2的增殖,其抑制效应与NO浓度呈正相关;合适浓度的外源性NO可在对细胞本身不产生明显毒性的情况下显著增强DDP对CNE-2细胞株的化疗敏感性。
Objective To investigate the effect of exogenous nitric oxide (NO) on the proliferation of CNE-2 cells induced by cisplatin (DDP), and to provide experimental and theoretical evidence for improving the efficacy of chemotherapy in nasopharyngeal carcinoma. Methods Nitric oxide sodium (SNP), DDP and their combination were used to treat CNE-2 cells. Nitric acid reductase method was used to detect the concentration of NO. Cell morphology was observed microscopically. CCK-8 assay Cell proliferation inhibition rate and flow cytometry apoptosis. Results (1) SNP inhibited CNE-2 cell proliferation and promoted apoptosis in a concentration-dependent manner. (2) There was a positive correlation between NO concentration and SNP concentration in supernatant (r = 0.968, P <0.001) (3) Compared with SNP group and DDP group, the morphology of CNE-2 cells in DDP + SNP combined group was significantly different, the number of floating cells was significantly increased, the number of adherent cells was gradually decreased and the original morphology was lost; (4) When SNP 600μmol / L (P <0.001). (5) The apoptosis rate of CNE-2 cells increased when combined with DDP in the SNP group, but not in the DDP group Compared with single use SNP, DDP group was significantly enhanced (t = -19.816, P <0.001; t = -5.242, P = 0.035). Conclusions Exogenous NO can inhibit the proliferation of CNE-2 and the inhibitory effect is positively correlated with the concentration of NO. Exogenous NO at a suitable concentration can significantly enhance the inhibitory effect of DDP on CNE-2 cell line Chemosensitivity.