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【目的】全基因组水平鉴定亚麻纤维素合酶超家族基因Ces A/Csls,并对基因的进化、基因结构及组织表达特性等进行分析,为亚麻纤维发育的机理研究奠定基础。【方法】利用Phytozome基因组数据库,通过生物信息学手段,鉴定亚麻纤维素合酶超基因家族成员,并进行蛋白理化特性分析;利用MEGA 5.0、GSDS、MEME等软件构建系统进化树,并进行基因结构、蛋白保守基序分析;根据RNA-Seq数据对Ces A/Csls进行表达分析。【结果】系统分析鉴定了45个亚麻Ces A/Csls超家族基因,该家族基因在scaffolds上是分散分布的,没有明显的成簇现象。Ces A/Csls蛋白主要分布于质膜上,氨基酸数目为409—1 167,分子量为47 401.1—130 578.3,等电点分布在5.43—9.08。Ces A/Csl蛋白均含有跨膜结构域,数目为2—8。根据系统进化分析将其分成Ces A与Csl两类,细分为Ces A、Csl A、Csl B、Csl C、Csl D、Csl E、Csl G共7组。基因结构分析显示,亚麻Ces A/Csls基因的长度在2.1—6.8 kb,外显子数量在2—14。保守基序分析表明,不同组间Motif组成有一定的差异,Motif 1、Motif 2、Motif 3、Motif 4、Motif 12在Ces A、Csl B、Csl D、Csl E、Csl G组蛋白中均有分布,Motif 18、Motif 20在Csl A、Csl C组蛋白中均有分布,而Motif 13、Motif 14、Motif 15、Motif 19的分布则表现出一定的组间特异性。表达谱分析结果表明,Ces A/Csls家族成员在不同发育阶段表达模式不同,部分Ces A/Csls可被Na Cl、BR和Brz诱导上调或下调表达,预示Ces A/Csls功能的多样性以及在植物发育过程中扮演着不同角色。【结论】鉴定出45个亚麻Ces A/Csls家族基因成员,分属于两类,7组,分布于scaffolds上,基因结构和蛋白基序具有组间多样性和组内保守性。不同的基因在不同发育阶段具有一定的时空特异性。Ces A/Csls中部分基因响应激素BR、Brz及Na Cl胁迫。
【Objective】 Ces A / Csls gene was identified at the whole genome level and the gene evolution, gene structure and tissue expression characteristics were analyzed, which laid the foundation for the study on the mechanism of flax fiber development. 【Method】 The phylogenetic tree of flax cellulose synthase was identified by bioinformatics methods using Phytozome genome database and phylogenetic analysis of protein was carried out. Phyto-phylogenetic tree was constructed by using software MEGA 5.0, GSDS and MEME, , Protein conserved motif analysis; Ces A / Csls expression analysis based on RNA-Seq data. 【Result】 Systematic analysis identified 45 Ces A / Csls superfamily genes of flax, which were scattered on scaffolds with no obvious clustering. Ces A / Csls protein mainly distributed on the plasma membrane, the number of amino acids 409-1 167, molecular weight 47 401.1-130 578.3, isoelectric point distribution at 5.43-9.08. Ces A / Csl proteins all contain a transmembrane domain with a number of 2-8. According to phylogenetic analysis, Ces A and Csl were divided into two groups, which were divided into Ces A, Csl A, Csl B, Csl C, Csl D, Csl E and Csl G. Gene structure analysis showed that the length of Ces A / Csls flax gene was 2.1-6.8 kb and the number of exons was 2-14. Motif 1, Motif 2, Motif 3, Motif 4 and Motif 12 in Motif 1, Csl B, Csl D, Csl E and Csl G histones were all conserved among the mothers Motif 18 and motif 20 were distributed in Csl A and Csl C histone proteins, while Motif 13, Motif 14, Motif 15 and Motif 19 showed a certain group specificity. The results of expression profiling showed that the expression patterns of Ces A / Csls family members differed at different developmental stages. Some Ces A / Csls could be upregulated or downregulated by Na Cl, BR and Brz, indicating the diversity of Ces A / Plant development plays different roles. 【Conclusion】 The 45 members of Ces A / Csls family of flax were identified. They belong to two groups and 7 groups, which are distributed on scaffolds. The gene structure and protein motif have diversity and intra-group conservation. Different genes have certain spatio-temporal specificity at different developmental stages. Some genes in Ces A / Csls responded to the stress of BR, Brz and NaCl.