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目的:建立RP-HPLC法同时测定生脉注射液中7个人参皂苷类成分(人参皂苷Rf、Rb1、Rg2、Rc、Rb2、Rb3和Rd)的含量。方法:使用Zorbax SB-C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-水为流动相梯度洗脱(0～15 min,29∶71;15～30min,33∶67),流速1.0 mL.min-1,检测波长203 nm,柱温30℃。结果:在本色谱条件下7个人参皂苷类成分之间有良好的分离度,各成分的浓度和相应的峰面积之间呈现良好的线性关系(r=0.9999,n=7),精密度、重复性及加样回收率的RSD均小于3%。结论:本方法可同时测定生脉注射液中7个人参皂苷类成分的含量,可作为生脉注射液的质量控制手段。
Objective: To establish an RP-HPLC method for the simultaneous determination of seven ginsenosides (ginsenosides Rf, Rb1, Rg2, Rc, Rb2, Rb3 and Rd) in Shengmai injection. Methods: The mobile phase was eluted with acetonitrile-water gradient (0-15 min, 29:71; 15-30 min, 33:67) using a Zorbax SB-C18 column (4.6 mm × 250 mm, 5 μm) mL.min-1, detection wavelength 203 nm, column temperature 30 ℃. Results: There was a good linear relationship between the concentration of the seven ginsenosides and the corresponding peak area (r = 0.9999, n = 7) under the chromatographic conditions. The precision, Repeatability and sample recovery RSD were less than 3%. Conclusion: The method can simultaneously determine the content of seven ginsenosides in Shengmai injection and can be used as quality control means of Shengmai injection.