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文冠果是中国北方特有的优良木本油料能源树种。利用生物信息学方法,将全长为1 491 bp的文冠果Xs FAE1基因与其他物种FAE1基因的核酸和氨基酸序列进行了比对分析。结果表明:(1)文冠果的第48位碱基产生了突变C→T,使得第16位氨基酸Asn→Tyr,第72位碱基C→T,使得第24位氨基酸Val→Leu,第845位T→C,使得第282位氨基酸Ser→Pro,第968位T→G,使得第323位氨基酸Ser→Ala;另外,文冠果Xs FAE1基因存在两个3 bp碱基缺失,分别为第165~167位和第1 169~1 171位,一个位于第1 517~1 518位的2 bp碱基缺失。(2)文冠果FAE1基因存在11个高芥酸植物特征的氨基酸保守位点,分别为6个半胱氨酸位点:Cys95、Cys224、Cys270、Cys312、Cys389和Cys460,4个组氨酸位点:His298、His387、His391和His420,以及283位的Ser。(3)文冠果属中芥酸植物源于两个高芥酸位点丝氨酸(Ser)的突变及一个第165~167位的3碱基(ATA)缺失。这可为研究文冠果芥酸的形成和积累提供参考,也可为开展文冠果低芥酸的分子育种提供基础。
Wen Guan fruit is unique to northern China woody energy tree species. Bioinformatics methods were used to compare the nucleotide and amino acid sequences of the Xs FAE1 gene of the full-length 1 491 bp with the FAE1 gene of other species. The results showed as follows: (1) Mutation C → T was found in the 48th base of Wen Guan Guo such that the 16th amino acid Asn → Tyr and the 72th base C → T, the 24th amino acid Val → Leu, At position 845 T → C, amino acid Ser → Pro at position 282 and T → G at position 968 resulted in amino acid Ser → Ala at position 323. In addition, there were two 3 bp base deletions in the Xs FAE1 gene The 165th to the 167th and the 1 169th to the 1 171th, and a 2 bp base deleted from the 1 517th to the 1 518th. (2) There are 11 amino acid conserved sites in FAE1 gene of Wen Guan Guo, including 6 cysteine sites: Cys95, Cys224, Cys270, Cys312, Cys389 and Cys460, 4 histidines Sites: His298, His387, His391 and His420, and Ser at position 283. (3) The Chinese erucic acid plant is derived from the mutation of the two high-erucic acid serines (Ser) and a third-base (ATA) deletion from the 165th to the 167th. This may provide a reference for the study of the formation and accumulation of Erucic acid, and also provide the basis for the molecular breeding of Erucic acid.