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为探讨肿瘤坏死因子 - α(TNF- α)诱导 K562 凋亡的作用 ,应用细胞形态学观察、DNA琼脂糖凝胶电泳 ,原位末端标记法检测 K562 、TNF-α诱导的 K562 。结果 表明 ,细胞形态学观察 K562 自然凋亡率 (2 .9± 0 .7) % ,TNF- α5 0 ng/ml、1 0 0 ng/ml诱导的 K562 凋亡率分别为 (1 6 .5± 4.1 ) % ,(2 3.9± 4.8) %。 (F=89.9,P<0 .0 1 )。 DNA琼脂糖凝胶电泳 TNF- α1 0 0 ng/ml诱导的 K562 DNA电泳呈梯状条带。原位末端标记法K562 自然凋亡率 (3.5± 1 .0 ) % ,TNF- α5 0 ng/ml、1 0 0 ng/ml诱导的 K562 凋亡率分别是(2 1 .4± 4.5 ) %、(2 7.4± 4.9) %。 (F=1 0 4.9,P<0 .0 1 )。结论 TNF-α 5 0 ng/ml、1 0 0 ng/ml能诱导 K562 凋亡
In order to investigate the role of tumor necrosis factor-α(TNF-α)-induced apoptosis in K562 cells, K562 and TNF-α-induced K562 were detected by cell morphology, DNA agarose gel electrophoresis and in situ end-labeling. The results showed that the natural apoptotic rate of K562 was (2.9 ± 0.7)%, and the apoptotic rates of K562 induced by TNF-α 50 ng/ml and 100 ng/ml were (16.5). ± 4.1 %, (2 3.9 ± 4.8) %. (F=89.9, P<0. 0 1 ). The electrophoresis of DNA by agarose gel electrophoresis showed a ladder-like banding of K562 DNA electrophoresis induced by TNF-α10 0 ng/ml. The intrinsic apoptotic rate (3.5±1.0%) of K562 in situ end labeling method, the apoptotic rates of K562 induced by TNF-α50 ng/ml and 100 ng/ml were (21.4±4.5)%, respectively. (2 7.4 ± 4.9)%. (F=1 0 4.9, P<0. 0 1 ). Conclusion TNF-α 50 ng/ml and 100 ng/ml can induce K562 apoptosis