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用２１０个１０碱基随机引物对玉米多胞质雄性不育系Ｍｏ１７－ＣＭＳ－１９Ａ（Ｔ组）、Ｍｏ１７－ＣＭＳ－Ｓｈａｎｇ２６（Ｓ组）、Ｍｏ１７－ＣＭＳ－Ｃ（Ｃ组）和它们的保持系Ｍｏ１７以及对新选育的尚未知其不育类型的糯玉米不育系Ｔａｉ－Ａ和它的保持系Ｃ１０３等６个品系的线粒体基因组进行了系统的ＲＡＰＤ分析。试验结果如下：（１）对其中４０个引物的扩增产物进行了聚类分析，绘制出６个品系的树式遗传关系图，并计算出了它们之间的遗传距离。其中，Ｔａｉ－Ａ和５个品系间关系较远，遗传距离为２４．９；Ｍｏ１７－ＣＭＳ－１９Ａ和Ｍｏ１７－ＣＭＳ－Ｃ间关系最近，其遗传距离为６。（２）用ＯＰＡＣ－０２、ＯＰＡＮ－０５、ＯＰＧ－１９、ＯＰＴ－０９和ＯＰＴ－１２等５种引物的扩增产物制作了６个品系的ＲＡＰＤ指纹，其中ＯＰＡＣ－０２６８０、ＯＰＡＣ－０２１０５３、ＯＰＡＮ－０５６８０、ＯＰＡＮ－０５３７０、ＯＰＴ－１２１２３０、ＯＰＴ－０９８００和ＯＰＧ－１９２９０结合起来可以分别作为鉴别Ｍｏ１７－ＣＭＳ－Ｓｈａｎｇ２６、Ｍｏ１７－ＣＭＳ－Ｃ、Ｍｏ１７－ＣＭＳ－１９Ａ、Ｔａｉ－Ａ、Ｍｏ１７、Ｃ１０３的分子标记，为快速、方便检测６个品系提供了可靠的?
A total of 210 10-mer random primers were used to analyze the effects of Mo17-CMS-19A (group T), Mo17-CMS-Shang26 (group S), Mo17- CMS-C Line Mo17 and mitochondrial genomes of 6 lines of newly mated waxy maize CMS line Tai-A and its maintainer line C103, which have not yet been well-known for their sterile types, were systematically analyzed by RAPD. The experimental results are as follows: (1) The amplification products of 40 primers were clustered and the tree genetic relationships of the six lines were drawn, and the genetic distance between them was calculated. Among them, Tai-A was distantly related to 5 lines and the genetic distance was 24.9. The relationship between Mo17-CMS-19A and Mo17-CMS-C was the closest, with a genetic distance of 6. (2) RAPD fingerprinting of six lines was made with the amplification products of OPAC-02, OPAN-05, OPG-19, OPT-09 and OPT-12. OPAC-02680, OPAC-021053, OPAN -05680, OPAN-05370, OPT-121230, OPT-09800, and OPG-19290, respectively, can be used as primers to identify Mo17- CMS-Shang26, Mo17- CMS- C, Mo17- CMS- 19A, Tai-A, Mo17, C103 Molecular markers provide reliable, fast and easy detection of 6 strains?