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确定了以啤酒废酵母为原料,酵母酶解液中α-氨基氮含量为考察指标的蜗牛酶酶解破壁条件;并以啤酒废酵母酶解液为有机氮源,厌氧发酵制备丁二酸。结果表明蜗牛酶破壁效果最好,其最佳反应条件为蜗牛酶用量10mg/g干物质,pH6.5,40℃水浴,反应16h,酵母酶解液中α-氨基氮含量为0.504g/100mL;当发酵培养基中葡萄糖浓度为50g/L,啤酒废酵母酶解液作为有机氮源时,菌株Actinobacillus succinogenes NJ113可以产34.6g/L丁二酸,收率69%,和以酵母膏为有机氮源厌氧发酵制备丁二酸(丁二酸浓度为35.1g/L,收率70.2%)比较,产量相近,成本较低。
Determine the enzymatic hydrolysis conditions of the snail enzyme using waste beer yeast as raw material and the α-amino nitrogen content in the yeast enzymatic hydrolyzate as the indicator; and use the beer waste yeast hydrolyzate as the organic nitrogen source to prepare Ding II acid. The results showed that snail was the best, the optimal reaction conditions were 10mg / g dry matter of snail enzyme, pH6.5, 40 ℃ water bath and reaction for 16h. The content of α-amino nitrogen in yeast hydrolyzate was 0.504g / 100mL. Actinobacillus succinogenes NJ113 could produce 34.6g / L succinic acid at a yield of 69% when the concentration of glucose in the fermentation medium was 50g / L and beer waste yeast hydrolyzate was used as organic nitrogen source. Anaerobic fermentation of organic nitrogen source succinic acid (succinic acid concentration of 35.1g / L, the yield of 70.2%) compared to yield similar, lower cost.