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以辣椒幼苗为试验材料,分别喷施不同浓度亚精胺、壳聚糖、水杨酸、CaCl2、甜菜碱溶液,采用昼温/夜温为10℃/5℃低温胁迫5天后进行相对电导率、冷害指数、可溶性糖含量、脯氨酸含量、丙二醛(MDA)含量等指标的测定和分析,探讨外源物质对低温胁迫下辣椒幼苗的影响及作用效果。结果表明,适宜浓度的外源物质可以显著降低辣椒幼苗低温胁迫后的相对电导率、冷害指数和MDA的积累量,提高可溶性糖、脯氨酸等渗透调节物质的含量,对提高辣椒幼苗抗冷性有积极作用。亚精胺、壳聚糖、水杨酸、CaCl2、甜菜碱提高辣椒幼苗抗冷性的最佳诱导浓度分别为0.5 mmol/L、50 mg/L、2 mmol/L、10 mmol/L、10 mmol/L。
Pepper seedlings were used as test materials, and different concentrations of spermidine, chitosan, salicylic acid, CaCl2 and betaine were sprayed on. The relative electrical conductivity was measured by using day temperature / night temperature of 10 ℃ / 5 ℃ for 5 days. , Chilling injury index, soluble sugar content, proline content, malondialdehyde (MDA) content and other indicators were measured and analyzed to explore the effects of exogenous substances on pepper seedlings under chilling stress and its effects. The results showed that the appropriate concentration of exogenous substances can significantly reduce the relative electrical conductivity, chilling injury index and MDA accumulation of pepper seedlings under chilling stress, and increase the contents of soluble sugar, proline and other osmoregulation substances, Sex has a positive effect. The optimum concentrations of spermidine, chitosan, salicylic acid, CaCl2 and betaine for improving chilling tolerance of pepper seedlings were 0.5 mmol / L, 50 mg / L, 2 mmol / L, 10 mmol / L, 10 mmol / L.