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本文用酶联免疫检测(ELISA)技术研究三叶一心期喷施AgNO3诱导苦瓜纯雌系完全花分化过程中花蕾与幼叶中内源激素含量变化。结果显示:喷施AgNO3后幼叶中IAA、GA3、ZR和ABA含量与喷水的相比,都是先下降后增加。AgNO3处理的花蕾中这4种激素含量在72h内没有一致变化规律,但变化幅度大于幼叶的,表明生殖器官的内源激素对苦瓜性别分化影响比营养器官的大。AgNO3处理后24～48h内,花蕾中这4种激素的含量明显低于喷水的,而其余时间则高于喷水的。此外,AgNO3处理的花蕾中ABA/IAA、ZR/IAA和GA3/IAA比值也在24～48h发生剧烈的变化,48h之后这些激素比值与喷水的相差不大。这些结果说明了AgNO3处理后24～48h是苦瓜纯雌系性别分化的关键时期,IAA可能是诱导纯雌系苦瓜雄性分化的关键激素。
In this paper, enzyme-linked immunosorbent assay (ELISA) technique was used to study the changes of endogenous hormones in flower buds and young leaves during the process of complete flower differentiation of pure female plantlets of Balsam pear. The results showed that the contents of IAA, GA3, ZR and ABA in young leaves sprayed with AgNO3 were the first to decrease and then increase. AgNO3-treated buds had no consistent change pattern within 72h, but the change range was larger than that of young leaves, indicating that the endogenous hormones of reproductive organs had a greater impact on sexual differentiation of vegetative parts than vegetative organs. AgNO3 treatment within 24 ~ 48h, the flower buds of these four hormones were significantly lower than the water spray, while the rest is higher than the water spray. In addition, the ratio of ABA / IAA, ZR / IAA and GA3 / IAA in flower buds treated with AgNO3 also varied drastically from 24 to 48 hours. After 48 hours, the ratio of these hormones to water spray did not differ significantly. These results indicate that 24-48 h after AgNO3 treatment is the key period for sex differentiation of pure female line of bitter melon, IAA may be the key hormone inducing male lineage differentiation of bitter melon.