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目的:分析评价芩连四物汤及其各部位对小鼠离体子宫收缩模型的影响,并探讨物质基础与效应之间的关系。方法:采用大孔吸附树脂法分离制备各部位样品(QL-1~QL-14);采用小鼠离体子宫收缩模型评价该方及各部位的生物效应;采用HPLC-DAD-ESI-MS法对活性显著部位QL-3中的主要色谱峰进行分析鉴定。结果:芩连四物汤拮抗小鼠离体子宫平滑肌收缩的主要活性部位是QL-1,QL-3,QL-5,QL-7,QL-11 5个部位;通过标准化合物对照及质谱特征对活性显著部位QL-3色谱峰进行了归属和指认,鉴定了其中29个化合物分别为苹果酸、没食子酸、梓醇、原儿茶酸、桃叶珊瑚苷、川芎嗪、香草酸、咖啡酸、芍药内酯苷、小檗红碱、芍药苷、四氢巴马汀、黄连碱、药根碱、益母草苷、甲基黄连碱、阿魏酸、巴马汀、小檗碱、野黄芩苷、黄芩素-7-O-葡萄糖苷、黄芩苷、地黄苷C、汉黄芩苷、白杨素-7-O-葡萄糖苷、千层纸苷、黄芩素、汉黄芩素、千层纸素-A。结论:芩连四物汤拮抗离体子宫收缩模型的主要活性部位为挥发油部位及去除大分子的醇沉上清液小分子部位;醇沉上清液小分子部位经大孔树脂吸附醇梯度洗脱得到了水洗脱部位,20%及60%醇洗脱3个活性部位。
Objective: To analyze and evaluate the effect of Qinlian Siwu Decoction and its various parts on the in vitro uterine contraction model in mice, and to explore the relationship between substance basis and effect. Methods: The macroporous adsorption resin was used to separate and prepare the samples of various parts (QL-1 ~ QL-14). The biological effects of this compound and its parts were evaluated by the mouse uterine contractions model. HPLC-DAD-ESI- The major chromatographic peak in QL-3 with significant activity was analyzed and identified. Results: The main active sites of QL-1, QL-3, QL-5, QL-7 and QL-11 were antagonized by Qinlian Siwu Decoction in vitro. The standard compound control and mass spectrometry Chromatographic peaks of QL-3 with significant activity were assigned and identified. Among them, 29 compounds were identified as malic acid, gallic acid, catalpol, protocatechuic acid, aucubin, ligustrazine, vanillic acid, caffeic acid , Paeoniflorin, berberine, paeoniflorin, tetrahydropalmatine, coptisine, jatrorrhizine, motherwort glycoside, berberine, ferulic acid, palmatine, berberine, , Baicalein-7-O-glucoside, baicalin, recuperazine C, baicalin, chrysin, chrysin, baicalein, wogonin, melatonin-A . CONCLUSION: The main active site of Qinlian Siwu decoction antagonizing uterine contraction model is the volatile oil fraction and the small molecule part of the alcohol precipitation supernatant for removing macromolecules. The small molecule part of the alcohol precipitation supernatant is subjected to macroporous resin adsorption alcohol gradient washing Take off the water off the site, 20% and 60% alcohol eluted three active sites.