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目的探讨创伤性脑损伤后大鼠海马microRNA的表达变化与认知功能障碍的相关性。方法 25只SD大鼠随机分为假手术组和伤后1h、1d、3d、5d组,采用Feeney自由落体法建立创伤性脑损伤动物模型。各组大鼠于设定的损伤时间点检测事件相关电位P300,应用基因芯片技术检测海马microRNA的表达情况,筛选特异性表达的microRNA。结果各组大鼠伤后P300的潜伏期延长,波幅下降,以伤后1d为甚。与假手术组相比,伤后各实验组microRNA表达谱具有显著差异。其中miR-21、miR-16、let-7b的表达与P300潜伏期变化存在显著相关,伤后1d为差异性表达的关键时间点。结论 microRNA可能在创伤性脑损伤后发生的认知功能障碍中发挥调控作用,有望为脑损伤后认知功能的法医学鉴定提供新的思路。
Objective To investigate the correlation between the changes of microRNA expression in hippocampus and cognitive impairment after traumatic brain injury in rats. Methods Twenty-five Sprague-Dawley rats were randomly divided into sham-operation group and 1h, 1d, 3d and 5d post-injury groups. Animal models of traumatic brain injury were established by Feeney free-fall method. The rats were sacrificed at the set time point to detect the event-related potential P300, and the microRNA expression of hippocampus was detected by microarray. Results The latency of P300 in rats in each group was prolonged and the amplitude was decreased, especially in the first day after injury. Compared with the sham-operated group, the expression of microRNA in each experimental group after injury was significantly different. Among them, the expression of miR-21, miR-16 and let-7b was significantly correlated with the change of P300 latency, and the critical time point of differential expression was 1d after injury. Conclusion MicroRNA may play a regulatory role in cognitive dysfunction after traumatic brain injury and may provide new ideas for forensic identification of cognitive function after brain injury.