月季组织培养法快速繁殖,已成为公认的高经济效益的手段。试管苗繁殖过程一般采用两步法:第一步培养增殖幼芽;第二步将增殖的幼芽转入长根培养基中,再将长根的试管苗从试管中取出移栽。此法处理手续繁杂。近两年来,我们试验成功试管繁殖一步法,即无根苗管外促根液处理法,具有成活率高、操作简便、省工、省时、省料等特点,进一步提高了经济效益。无根试管苗移栽技术如下。 (一)基质:月季虽对土壤要求不严,但以疏松,富含有机质,微酸性为最佳。因为是转栽无根试管 Rose tissue culture rapid propagation has become recognized as a means of high economic efficiency. Test tube seedling propagation generally use two-step method: the first step to cultivate proliferative shoots; the second step is to proliferate the shoots into rooting medium, and then remove the long-rooted tube seedlings from the test tube. This method is complicated procedures. In the past two years, we successfully tested one-step in vitro test tube propagation, that is, root exudation treatment without root canal, which has the characteristics of high survival rate, easy operation, labor saving, time saving and material saving, further improving economic benefits. Rootless tube seedlings transplanting techniques are as follows. (A) matrix: Although the lack of strict requirements of the soil, but loose, rich in organic matter, slightly acidic is the best. Because it is transplanted rootless test tube