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为探讨儿童幽门螺杆菌感染的可能途径,对198例儿童及69例一级亲属 ,采用PCR方法扩增唾液和胃粘膜H.pylori尿素酶C(UreC)基因(820bp) ,扩增产物分别用限制性核酸内切酶HhaI与AluI酶切消化 ,得出H.pylori的酶切类型(RFLPc)。结果显示 ,H.pyloriUreC基因扩增片断经限制性核酸内切酶HhaI与AluI酶切后 ,分别得到9种和7种不同的酶切图谱 ,结合两种酶切类型共确定22个独立的酶切类型 ;在31个儿童与亲属同时感染的家庭中 ,25.0 %(7/28例)的儿童唾液中可扩增出H.pylori,且唾液与相应胃粘膜中的幽门螺杆菌RFLPc酶切类型一致 ;83.9%(26/31例)儿童感染的H.pylori与其亲属感染的H.pylori类型相同 ,16.1%(5/31例)不相同(P<0.05)。表明PCR_RFLP分析H.pyloriUreC基因的酶切类型分辨率较高 ,直接扩增样本H.pylori方法简便 ,可用于H.pylori的流行病学研究 ;唾液可作为家庭内成员H.pylori感染的传播因素。
To explore the possible path of Helicobacter pylori infection in children, 198 cases of children and 69 first-degree relatives were enrolled in this study. The UreC gene (820bp) was amplified from the saliva and gastric mucosa by PCR. Restriction endonucleases HhaI and AluI digestion, resulting in H.pylori digestion type (RFLPc). The results showed that nine and seven different digestion patterns were obtained by restriction endonuclease HhaI and AluI, respectively. Two restriction enzyme digestion patterns were used to identify 22 independent enzymes Among the 31 children and their relatives infected simultaneously, H.pylori was amplified in the saliva of 25.0% (7/28) of children, and saliva was associated with the type of H.pylori RFLPc digested in the gastric mucosa . H.pylori infection in 83.9% (26/31) children was the same as that of their relatives (16.1% (5/31)) (P <0.05). PCR_RFLP analysis of H.pyloriUreC gene cleavage type resolution is higher, direct amplification sample H.pylori method is simple, can be used for epidemiological studies of H.pylori; saliva can be used as family members of H.pylori infection transmission factor .