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目的 通过检测鼻咽癌患者外周血单个核细胞中P-糖蛋白(P-gp)和肺耐药相关蛋白(LRP)的表达,探讨两者在鼻咽癌多药耐药中的作用及意义。方法 采用流式细胞术对54例鼻咽癌患者化疗前后外周血单个核细胞中P-gp、LRP进行检测,同期选择鼻咽部非恶性肿瘤患者10例,正常健康人10例为对照组。结果 P-gp在正常人、非恶性肿瘤及鼻咽癌初治组中的表达差异无显著性,但在复发组的表达明显高于其他组,差异有显著性。LRP在初治及复发组中的表达明显高于对照组,且化疗前后差异有显著性。P-gp和LRP的表达与年龄、性别、临床分期、病理类型、淋巴结转移无显著相关。P-gp、LRP的表达与化疗疗效密切相关。结论 P-gp、LRP的表达在鼻咽癌多药耐药的产生中具有重要意义,对临床制定化疗方案具有潜在的指导意义。
Objective To detect the expression of P-gp and LRP in peripheral blood mononuclear cells of patients with nasopharyngeal carcinoma (NPC), and to explore their roles and significance in multidrug resistance of nasopharyngeal carcinoma . Methods Flow cytometry was used to detect the levels of P-gp and LRP in peripheral blood mononuclear cells of 54 patients with nasopharyngeal carcinoma before and after chemotherapy. Ten patients with nasopharyngeal non-malignant tumor and 10 normal controls were selected as the control group. Results There was no significant difference in the expression of P-gp between normal controls, non-malignant tumors and primary nasopharyngeal carcinoma patients, but the expression of P-gp in the relapsed group was significantly higher than that in other groups. The expression of LRP in the naive and recurrent group was significantly higher than that in the control group, and the difference was significant before and after chemotherapy. P-gp and LRP expression and age, gender, clinical stage, pathological type, lymph node metastasis was not significantly correlated. P-gp, LRP expression is closely related to the efficacy of chemotherapy. Conclusion The expression of P-gp and LRP is of great importance in the development of multi-drug resistance in nasopharyngeal carcinoma, and has potential guiding significance for the development of chemotherapy regimen.