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目的:研究抗凋亡基因Bag-1在乳腺癌癌前病变组织中的表达及Bcl-2、Bax的表达情况,探讨Bag-1与Bcl-2、Bax的相互关系及其临床意义。方法:采用链霉菌抗生物素蛋白-过氧化物酶(S-P)免疫组化方法对15例乳腺平坦型上皮不典型增生(FEA)、20例乳腺导管内癌(DCIS)、20例乳腺浸润性导管癌(IDC)及20例乳腺正常组织中Bag-1、Bcl-2、Bax的表达进行检测并收集全部患者的临床病理资料进行统计学分析。结果:①Bag-1、Bcl-2、Bax在15例乳腺FEA、20例乳腺DCIS、20例乳腺IDC及20例乳腺正常组织中的阳性表达率分别为33.3%、33.3%、20.0%;70.0%、30.0%、50.0%;70.0%、50.0%、65.0%;25.0%、30.0%、10.0%。②Bag-1在乳腺FEA中的阳性表达率低于乳腺IDC和DCIS中的表达(P<0.05),Bag-1在乳腺正常组织中的阳性表达率低于乳腺IDC和DCIS中的表达(P<0.01);Bax在乳腺FEA中的阳性表达率低于乳腺IDC中的表达(P<0.01),Bax在乳腺正常组织中的阳性表达率低于乳腺IDC和DCIS中的表达(P<0.01)。③在乳腺FEA中Bag-1与Bcl-2的阳性表达呈正相关关系(P<0.01),在乳腺DCIS中Bag-1与Bax的阳性表达、Bcl-2与Bax的阳性表达呈正相关关系(P<0.01)。结论:检查结果说明在肿瘤发生发展的多基因调节中,Bag-1与Bcl-2在从FEA到癌变的过程中有相互促进作用。肿瘤的发生发展过程是受多基因调控的,多因素共同影响肿瘤的增殖。
Objective: To study the expression of Bcl-2 and Bax in anti-apoptotic gene Bag-1 in precancerous lesions of breast cancer and to explore the correlation between Bag-1, Bcl-2 and Bax and its clinical significance. Methods: Streptomyces avidin - peroxidase (SP) immunohistochemical method was used to detect 15 cases of breast flat epithelial dysplasia (FEA), 20 cases of intraductal carcinoma (DCIS), 20 cases of invasive breast (IDC) and Bag-1, Bcl-2 and Bax expression in 20 normal breast tissues were collected and the clinical and pathological data of all patients were collected for statistical analysis. RESULTS: ① The positive rates of Bag-1, Bcl-2 and Bax were 33.3%, 33.3% and 20.0% in 15 cases of breast FEA, 20 cases of breast DCIS, 20 cases of breast IDC and 20 cases of breast normal tissues respectively. The positive rates of Bax, , 30.0%, 50.0%; 70.0%, 50.0%, 65.0%; 25.0%, 30.0%, 10.0%. ② The positive expression rate of Bag-1 in breast FEA was lower than that in breast IDC and DCIS (P <0.05). The positive expression rate of Bag-1 in normal breast tissue was lower than that in breast IDC and DCIS (P < 0.01). The positive expression rate of Bax in breast FEA was lower than that in breast IDC (P <0.01). The positive expression rate of Bax in breast normal tissue was lower than that in breast IDC and DCIS (P <0.01). (3) The positive expression of Bag-1 and Bcl-2 in breast FEA was positively correlated (P <0.01). The positive expression of Bag-1 and Bax in breast DCIS was positively correlated with the positive expression of Bcl-2 and Bax (P <0.01). Conclusions: The results of the examination show that Bag-1 and Bcl-2 promote each other in the process of carcinogenesis from FEA in the multi-gene regulation of tumorigenesis. The occurrence and development of tumors are regulated by multiple genes, and many factors affect the proliferation of tumors together.