论文部分内容阅读
为研究甲基化CpG结合域蛋白2(methyl-CpG binding domain protein 2,MBD2)在围植入期小鼠子宫内膜的表达规律,通过采用实时荧光定量PCR(Real-time fluorescence quantitative PCR,qPCR)、Western blot和免疫组化技术检测未孕小鼠(d0)和不同孕天小鼠子宫MBD2的表达情况。qPCR结果显示,d0至d7的小鼠子宫内膜组织均有MBD2 mRNA表达,在d5至d7高表达。MBD2蛋白在子宫内膜的表达规律与qPCR结果相符。MBD2蛋白在孕d1到d4中度表达于腔上皮、腺上皮和基质细胞,在d5至d7基质细胞表达增强,主要表达于蜕膜区。假孕小鼠子宫内膜中,MBD2在腔上皮、腺上皮和基质细胞中中度表达,d5至d7基质细胞表达明显减弱。动物模型中,宫角注射MBD2基因反义寡聚脱氧核苷酸,可抑制MBD2的表达,降低人工诱导蜕膜化反应和蜕膜化标志物PRL的表达。MBD2在早孕小鼠子宫内膜的表达模式提示其可能参与了蜕膜化过程。
In order to study the expression of MBD2 in mouse endometrium during peri-implantation period, real-time fluorescence quantitative PCR (qPCR) ), Western blot and immunohistochemistry were used to detect the expression of MBD2 in uterus of non-pregnant mice (d0) and different pregnant mice. qPCR showed that MBD2 mRNA was expressed in mouse endometrium from d0 to d7 and was highly expressed from d5 to d7. MBD2 protein expression in the endometrium consistent with the qPCR results. MBD2 protein was moderately expressed in luminal epithelial cells, glandular epithelium and stromal cells from d1 to d4 during pregnancy, and increased in d5 to d7 stromal cells, mainly in the decidua area. Among the pseudopregnant mouse endometrium, MBD2 was moderately expressed in luminal epithelium, glandular epithelium and stromal cells, and d5 to d7 stromal cells were significantly decreased. In animal model, intrauterine injection of MBD2 gene antisense oligodeoxynucleotides inhibits the expression of MBD2 and reduces the expression of PRL induced by artificially induced decidualization and decidualization. The expression pattern of MBD2 in early pregnancy mouse endometrium suggests that it may participate in the decidualization process.