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进行2个试验考察饲料中添加甘露寡糖对奥尼罗非鱼(Oreochromis niloticus×O.aureus)生长、肠道结构功能和血清非特异性免疫的影响.在基础饲料中分别添加0.00%(对照组)、0.25%、0.50%、0.75%甘露寡糖,饲养平均初重(1.00±0.04)g的奥尼罗非鱼8周(试验Ⅰ),或饲养平均初重(20.04±0.13)g的奥尼罗非鱼30d(试验Ⅱ).结果表明,在2个试验中0.50%、0.75%甘露寡糖组均显著提高鱼体增重率,降低饲料系数(P<0.05);试验Ⅱ中,0.25%、0.50%、0.75%甘露寡糖组均显著提高小肠绒毛高度、宽度、密度,降低肠壁厚度,提高胃、肠蛋白酶活性和干物质消化率,提高血清超氧化物歧化酶(SOD)活性和碱性磷酸酶活性(P<0.05),但对小肠绒毛杯状细胞数量无显著影响(P>0.05).上述研究表明,饲料中添加0.50%～0.75%甘露寡糖可显著提高罗非鱼生长性能,改善肠道结构功能,提高营养物质消化率和机体非特异性免疫功能.奥尼罗非鱼饲料中的甘露寡糖适宜添加量推荐为0.50%.
Two experiments were conducted to investigate the effects of adding mannooligosaccharides to feed on Oreochromis niloticus × O. aureus growth, intestinal structure function and serum non-specific immunity.On the basis of feed, 0.00% (control group ), 0.25%, 0.50%, 0.75% mannooligosaccharides, fed with O. niloticus with an average initial weight of (1.00 ± 0.04) g for 8 weeks (testⅠ), or fed nilotinol with an average initial weight of (20.04 ± 0.13) g The results showed that 0.50% and 0.75% mannose oligosaccharides significantly increased the weight gain of fish and decreased the feed efficiency (P <0.05) in the two experiments. In experiment Ⅱ, 0.25% 0.50% and 0.75% mannose oligosaccharides significantly increased the villus height, width and density, decreased the intestinal wall thickness, increased the activity of gastric and intestinal protease and the digestibility of dry matter, and increased the activities of serum superoxide dismutase (SOD) and base (P <0.05), but had no significant effect on the number of goblet goblet cells (P> 0.05) .This study showed that the addition of 0.50% ~ 0.75% mannooligosaccharides to feed significantly increased the growth performance of tilapia , Improve intestinal structure and function, improve nutrient digestibility and non-specific immune function of the body. Mannan oligosaccharides feed appropriate to add the recommended amount of 0.50%.