采用反相高效液相色谱法测定了米根霉乳酸发酵液中的乳酸。在Wakosil Ⅱ 5C18RS (4 6mmi d ×15 0mm ,5 μm)色谱柱上 ,以 0 0 1mol/L磷酸溶液 (pH 2 5 )作流动相 ,流速为 1mL/min ,紫外检测波长为 2 10nm。实验结果表明 ,该方法的相对标准偏差为 0 2 2 % (n =5 ) ,回收率为 99%以上。方法简便、快速、可靠 Determination of lactic acid in Rhizopus oryzae lactic acid fermentation broth by reversed-phase high performance liquid chromatography. The mobile phase was a 0 0 1 mol / L phosphoric acid solution (pH 2 5) with a flow rate of 1 mL / min and a UV detection wavelength of 210 nm on a Wakosil Ⅱ 5C18RS column (4.6 mm × d 150 mm, 5 μm). The experimental results show that the relative standard deviation of the method is 0 2 2% (n = 5) and the recovery rate is above 99%. The method is simple, fast and reliable