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目的探讨甲状腺乳头状癌中MRP-1基因mRNA和蛋白的表达及其临床意义。方法采用逆转录多聚酶链反应(RT-PCR)和免疫组织化学(EnvisionTM)法,检测36例甲状腺乳头状癌组织标本中MRP-1基因mRNA和蛋白的表达,并结合临床资料进行分析。结果MRP-1的免疫组化和RT-PCR的检测结果相符(P<0.01)。甲状腺乳头状癌中MRP-1 mRAN表达水平为1.41±0.47,蛋白阳性率为36.1%(13/36),两者均低于正常甲状腺组织、结节性甲状腺肿及滤泡性腺瘤(P<0.05);MRPl基因mRNA和蛋白表达与甲状腺乳头状癌侵袭转移有关(P<0.05)。结论MRP-1基因与甲状腺乳头状癌的发生、发展、侵袭转移及预后有关。
Objective To investigate the expression of MRP-1 mRNA and protein in thyroid papillary carcinoma and its clinical significance. Methods The mRNA and protein expression of MRP-1 in 36 samples of papillary thyroid carcinoma were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry (EnvisionTM), and analyzed by clinical data. Results The results of MRP-1 immunohistochemistry and RT-PCR were consistent (P <0.01). The MRP-1 mRAN expression in papillary thyroid carcinoma was 1.41 ± 0.47 and the protein positive rate was 36.1% (13/36), both of which were lower than those in normal thyroid tissue, nodular goiter and follicular adenoma (P < 0.05). The mRNA and protein expression of MRP1 gene was correlated with invasion and metastasis of papillary thyroid carcinoma (P <0.05). Conclusion MRP-1 gene is associated with the occurrence, development, invasion, metastasis and prognosis of papillary thyroid carcinoma.