论文部分内容阅读
目的检测某部采购食品中黄曲霉毒素B1的含量,了解黄曲霉毒素B1在不同种类食品间的分布情况。方法选取5个食堂3大类食品(谷物、植物油和酿造类)共计68份样本,用酶联免疫吸附法(ELISA)进行定量测定,用统计学方法对数据结果进行分析。结果黄曲霉毒素B1 ELISA标准曲线相关系数r在0.99以上,样本加标回收率在96.22%~115.09%之间;68份样本合格率为98.53%;谷物、植物油和酿造类食品黄曲霉毒素B1含量均值分别为0.168 7、0.125 7、0.323 1μg/kg,差异有统计学意义(F=16.96,P<0.01),其中谷物类食品中黄曲霉毒素B1含量显著高于其他两类。结论该部采购食品中黄曲霉毒素B1含量合格率高,但仍有个别食品存在安全隐患,应加强食品采购、储存和加工等各个环节的监督管理,重视食品安全监测。
Objective To detect the content of aflatoxin B1 in a certain purchased food and to understand the distribution of aflatoxin B1 in different kinds of food. Methods A total of 68 samples from 3 canteens (cereal, vegetable oil and brewing) in 5 canteens were selected and quantified by enzyme-linked immunosorbent assay (ELISA). The data were analyzed by statistical methods. Results The correlation coefficient r of the standard curve of aflatoxin B1 was above 0.99, the spiked recoveries of samples were between 96.22% and 115.09%, the pass rate of 68 samples was 98.53%, the content of aflatoxin B1 in cereal, vegetable oil and brewed food The mean values were 0.168 7,0.125 7,0.323 1μg / kg respectively, the difference was statistically significant (F = 16.96, P <0.01). The content of aflatoxin B1 in cereal foods was significantly higher than the other two categories. Conclusion The Department of Food procurement aflatoxin B1 content pass rate, but there are still some food safety problems, should strengthen food procurement, storage and processing of all aspects of supervision and management, emphasis on food safety monitoring.