以往许多学者曾用传统的组织切片法进行了这方面的研究。Engstrm等(1966)用耳蜗铺片法观察了内耳神经上皮组织状态,作者将豚鼠的小脑前下动脉阻断后,用铺片法观察螺旋器的受损部位及范围。实验目的,想佐证美尼尔氏病病源之一是由于内耳血循环障碍之说。用41只年青体壮的豚鼠为实验对象,动物标本的制备,按Engstrm氏所述方法。分别在术后3、6、12、18、24小时及3、4、5、7、8、14、21及28天处死动物,用位相显微镜从基 In the past, many scholars used the traditional tissue slicing method in this aspect. Engstrm et al (1966) observed the inner ear nerve epithelial tissue state using the cochlear flap method, the author of guinea pig occlusion of the anterior inferior cerebellar artery, using the laying method to observe the damaged parts and extent of the spiral. Experimental purposes, to prove one of the sources of Meniere’s disease is due to the inner ear blood circulation disorders. 41 young and healthy guinea pigs as experimental subjects, the preparation of animal specimens, according to Engstrm’s method described. Animals were sacrificed at 3, 6, 12, 18, 24 hours and 3, 4, 5, 7, 8, 14, 21 and 28 days after surgery respectively.