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对鼠李糖脂生产菌种假单胞菌Pseudomonas sp. sy3进行自身产物诱变选育高产菌株,并对生产鼠李糖脂的培养基和发酵条件进行了研究。经过鼠李糖脂产物诱变,获得1株鼠李糖脂产量较高的正突变株322,该突变菌株鼠李糖脂产量达到27.5 g/L,比出发菌株提高了57.14%。
The rhamnolipid-producing strain Pseudomonas sp. Sy3 was inbred by self-made high-yielding strain, and the production of rhamnolipid medium and fermentation conditions were studied. After mutagenesis of rhamnolipid, one positive mutant strain 322 with higher rhamnolipid yield was obtained. The rhamnolipid production of this mutant strain reached 27.5 g / L, which was 57.14% higher than that of the original strain.